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When the immunoprecipitate is fully reduced immediately prior to SDS-gel electrophoresis, reactivity of Mouse IgG TrueBlot ULTRA with the 55 kDa heavy chains and the 23 kDa light chains of the immunoprecipitating antibody is minimized thereby eliminating interference by the heavy and light chains of the immunoprecipitating antibody in IP/immunoblot applications. TrueBlot preferentially detects the non-reduced form of mouse IgG over the reduced, SDS-denatured form of IgG. Mouse IgG TrueBlot ULTRA is ideal for use in protocols involving immunoblotting of immunoprecipitated proteins. Western blot detection of immunoprecipitated proteins is a commonly used technique to study protein-protein interactions and immunoprecipitation (IP) is. It is easy to generate publication-quality IP/WB data with Mouse IgG TrueBlot® ULTRA. Use it in place of your conventional HRP Anti-Mouse IgG immunoblotting secondary antibody. Mouse IgG TrueBlot® ULTRA is the unique horseradish peroxidase conjugated Anti-Mouse IgG imonoclonal secondary antibody which enables detection of immunoblotted target protein bands, without hindrance by interfering immunoglobulin heavy and light chains from your IP antibody. Western blotting, cyclin-dependent kinases. The TrueBlot® Immunoprecipitation and Western Blot Kit for DYKDDDDK (FLAG®) Epitope Tag contains the critical supporting reagents, buffers, and substrates for immunoprecipitation and Western blotting of samples containing the DYKDDDDK (FLAG®) Epitope Tag using TrueBlot monoclonal secondary antibody in conjunction with Rockland's Antibody for the detection of FLAG™ conjugated proteins (MOUSE) Monoclonal Antibody (see application_notes). Terms to be familiar with before you start to solve the test: immunoprecipitation, affinity chromatography. Antigens A, B, and C generated polyclonal antibodies (pAb) A302-010A, A302-011A, and A302- 012A respectively. Three distinct peptides representing epitopes of beta-catenin were synthesized as antigens for polyclonal antibody production in rabbits (A, B, C). The Anti-DYKDDDDK (FLAG®) antibody included in this Western blot kit detects FLAG®-tagged recombinant proteins fused with FLAG® tag either at the amino-terminus or carboxy-terminus of the protein. A western blot/immunoprecipitation assay verifies antibody specificity. The hydrophilic character of this sequence increases the likelihood that it will be located on the surface of FLAG®-tagged recombinant proteins for reaction with anti-DYKDDDDK (FLAG®) antibodies. The FLAG® epitope is a small but highly immunogenic peptide DYKDDDDK (N-Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys-C) which allows fusion proteins to retain their original conformation and function.